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Brief Report: A Blood-Based MicroRNA Complementary Diagnostic Predicts Immunotherapy Efficacy in Advanced-Stage NSCLC With High Programmed Death-Ligand 1 Expression
Institute of Pathology, Heidelberg University Hospital, Heidelberg GermanyTranslational Lung Research Center (TLCR) at Heidelberg University Hospital, Member of the German Center for Lung Research (DZL), Heidelberg, Germany
LungenClinic Grosshansdorf, Airway Research Center North, German Center for Lung Research (DZL), Grosshansdorf, GermanyDepartment of Medicine, Christian Albrechts University of Kiel, Kiel, Germany
Translational Lung Research Center (TLCR) at Heidelberg University Hospital, Member of the German Center for Lung Research (DZL), Heidelberg, GermanyDepartment of Thoracic Oncology, Thoraxklinik and National Center for Tumor Diseases (NCT) at Heidelberg University Hospital, Heidelberg, Germany
Translational Lung Research Center (TLCR) at Heidelberg University Hospital, Member of the German Center for Lung Research (DZL), Heidelberg, GermanyDepartment of Thoracic Oncology, Thoraxklinik and National Center for Tumor Diseases (NCT) at Heidelberg University Hospital, Heidelberg, Germany
Corresponding author. Address for correspondence: Bruno R. Steinkraus, PhD, Hummingbird Diagnostics GmbH, Im Neuenheimer Feld 583, 69120 Heidelberg, Germany.
Patients with advanced, non-oncogene-driven NSCLC with high programmed death-ligand 1 (PD-L1) expression are eligible for treatment with immunotherapy. There is, however, an urgent medical need for biomarkers identifying cases that require additional combination with chemotherapy. We previously uncovered a myeloid-based 5-microRNA (miRNA) signature that identified responders to immunotherapy in PD-L1 unstratified patients; however, its potential utility in treatment guidance for patients with PD-L1 high tumors remained unclear.
Methods
We trained (n = 68) and validated (n = 56) a 5-miRNA multivariable Cox proportional hazards model predictive of overall survival on small RNA sequencing data of whole blood samples prospectively collected before the commencement of immunotherapy for stage IV NSCLC with PD-L1 tumor proportion score greater than or equal to 50%, treated with PD-1 inhibitor monotherapy (immunotherapy alone [IO]). Specificity was demonstrated in a control cohort treated with immunochemotherapy (ICT) (n = 31).
Results
The revised 5-miRNA risk score (miRisk) stratified IO-treated patients and identified a high-risk group with significantly shorter overall survival (hazard ratio = 5.24, 95% confidence interval: 2.17–12.66, p < 0.001). There was a significant interaction between the miRisk score and type of treatment (IO or ICT, p = 0.036), indicating that the miRisk score may serve as a predictive biomarker for immunotherapy response. Furthermore, the miRisk score could identify a group of high-risk patients who may benefit from treatment with ICT as opposed to IO (hazard ratio = 0.35, 95% confidence interval: 0.15–0.82, p = 0.018).
Conclusions
The miRisk score can distinguish a group of patients with PD-L1 high, stage IV NSCLC likely to benefit from adding chemotherapy to immunotherapy and may support treatment decisions as a blood-based complementary diagnostic.
Immunotherapy is revolutionizing the standard of care for numerous cancers; however, an important limitation is the lack of reliable efficacy biomarkers. In advanced-stage NSCLC expressing high levels of programmed death-ligand 1 (PD-L1) (PD-L1 tumor proportion score [TPS] ≥ 50%), both immunotherapy alone (IO) or in combination with chemotherapy (ICT) are recommended as treatment options in major international guidelines.
Nevertheless, in clinical reality, these two options may not be equal, and there are patients unresponsive to IO monotherapy, who need additional chemotherapy. So far, no results from prospective trials can support decision-making on this clinically important issue and there is an unmet need for therapy selection biomarkers.
A positive response to immunotherapy is dependent both on local interactions between cancer and immune cells in the tumor microenvironment
Defining the latter has the potential to provide noninvasive therapy guidance, but no blood-based biomarkers have yet entered routine clinical use.
We previously reported the development of a circulating myeloid cell-derived 5-microRNA (miRNA) signature — miRisk — to predict overall survival (OS) of patients with advanced NSCLC treated with IO in PD-L1 TPS unstratified patients.
Here, we systematically evaluated the ability of a revised miRisk score to identify PD-L1 high patients who are likely to benefit from chemotherapy in addition to immunotherapy.
Materials and Methods
This study included a total of 155 prospectively recruited patients with stage IV NSCLC and PD-L1 TPS greater than or equal to 50% whose blood samples were collected before immunotherapy treatment (2017–2020). Patients with actionable mutations in EGFR, ALK, and ROS1 were identified by combined DNA and RNA next-generation sequencing
and excluded from this study. All patients provided written informed consent. Samples were obtained from the Lungenbiobank Heidelberg and Biobank Nord within the German Center for Lung Research (DZL) according to the pertinent regulations after approval of the ethics committees at Heidelberg University (S-296/2016, S-089/2019) and LungenClinic Grosshansdorf (AZ 12-238, AZ 19-286). Sample processing and generation of small RNA expression profiles are described in detail in our previous work.
All anonymized small RNA sequencing data have been deposited at the European Nucleotide Archive under accession number PRJEB50502. Survival analyses were performed in Python (3.8.8), using the packages scikit-survival (version 0.15.1)
miRisk low and high groups were defined based on the median risk score within the training cohort (low risk ≤ −0.0725 < high risk). Relevant clinical confounders to include in multivariable models were selected in consultation with a panel of thoracic oncologists (P.C., M.T., M.R.). Visualization was performed in GraphPad Prism (version 9.3.1, GraphPad Software).
Results
This study included 155 patients with stage IV NSCLC, divided into training (n = 68) and validation (n = 56) cohorts treated with IO, and split according to the time-point of sample collection, and a control cohort treated with ICT (n = 31) (Table 1). These three cohorts had similar clinicopathologic characteristics. Fitting a multivariable Cox proportional hazards model to the training cohort using the five previously identified miRNAs as input features led to a revised linear predictor risk score: miRisk = (ln(miR-2115-3p RPM + 1) × 2.190467) + (ln (miR-218-5p RPM + 1) × 0.303095) + (ln (miR-224-5p RPM + 1) × 0.598415) + (ln (miR-4676-3p RPM + 1) × 1.101122) + (ln (miR-6503-5p RPM + 1) × 0.958823).
We observed significantly longer OS in the miRisk-low patients in the training cohort (hazard ratio [HR] = 3.65, 95% confidence interval [CI]: 1.84–7.24, p < 0.001) (Supplementary Fig. 1). This finding was confirmed in the validation cohort (HR = 5.24, 95% CI: 2.17–12.66, p < 0.001) (Fig. 1A). In contrast, the miRisk score was not associated with OS in patients in the ICT control cohort (HR = 1.40, 95% CI: 0.21–9.28, p = 0.753) (Fig. 1B), consistent with previous reports that IO-specific biomarkers do not predict response to ICT.
Association of the advanced lung cancer inflammation index (ALI) with immune checkpoint inhibitor efficacy in patients with advanced non-small-cell lung cancer.
Figure 1OS of patients with NSCLC stratified by miRisk. (A, B) Comparison of OS between miRisk low and high groups in IO validation (n = 56) and the ICT control cohorts (n = 31). Significant differences in OS are observed in the validation but not the control cohort. (C, D) Comparison of OS between IO and ICT in miRisk-stratified cohorts. HR and 95% CIs were calculated using a univariable Cox regression analysis; p values were calculated using the log-rank test. All statistical analyses were two-sided. CI, confidence interval; HR, hazard ratio; ICT, immunochemotherapy; IO, immunotherapy alone; miRisk, 5-microRNA risk score; OS, overall survival.
To explore the utility of the miRisk score as a complementary diagnostic for IO versus ICT treatment decisions, we merged the validation and control cohorts to explore the interaction between the biomarker and type of treatment in terms of relationship with OS. In the miRisk-low patients, there was no difference in OS between those treated with IO or ICT (HR = 1.22, 95% CI: 0.12–12.25, p = 0.849) (Fig. 1C). In contrast, in miRisk-high patients, we observed significantly improved OS in patients receiving ICT compared with IO monotherapy (HR = 0.35, 95% CI: 0.15–0.82, p = 0.018) (Fig. 1D). The interaction between treatment and miRisk score in a multivariable Cox proportional hazards model was significant (HR = 1.62, 95% CI: 1.03–2.55, p = 0.036), suggesting the miRisk score is both prognostic and predictive for the efficacy of IO
Finally, we used multivariable Cox proportional hazards models to investigate the performance of the miRisk score when controlling for other relevant clinicopathological covariates. This revealed that the miRisk score had a stronger association with OS in IO-treated patients (HR = 3.82, 95% CI: 1.29–11.30, p = 0.015) than PD-L1 TPS, histologic subtype, Eastern Cooperative Oncology Group performance status, and therapy line (Table 2).
Table 2Univariable and Multivariable Cox Regression Analysis of miRisk and Clinical Covariates
Overall Survival
Univariable Analysis
Multivariable Analysis
Covariate
HR
95% CI
p Value
HR
95% CI
p Value
IO training cohort
ECOG performance status
1.39
0.76–2.52
0.281
0.87
0.46–0.51
0.682
Histologic subtype (nonadeno vs. adeno)
1.09
0.55–2.17
0.806
1.40
0.69–1.04
0.354
Therapy line
0.75
0.38–1.49
0.409
0.79
0.38–0.49
0.532
PD-L1 TPS
0.99
0.97–1.01
0.433
0.96
0.93–0.01
0.006
miRisk (high vs. low)
3.84
1.86–7.95
<0.001
7.41
2.95–2.93
<0.001
IO validation cohort
ECOG performance status
3.53
1.55–8.05
0.003
3.32
1.24–2.18
0.017
Histologic subtype (nonadeno vs. adeno)
1.89
0.79–4.50
0.151
1.79
0.69–1.54
0.235
Therapy line
1.57
1.05–2.34
0.029
1.23
0.81–0.63
0.335
PD-L1 TPS
1.00
0.97–1.03
0.753
1.00
0.97–0.04
0.848
miRisk (high vs. low)
5.37
1.96–14.74
0.001
3.82
1.29–2.42
0.015
ICT control cohort
ECOG performance status
3.70
0.85–16.06
0.081
6.15
1.00–37.99
0.050
Histologic subtype (nonadeno vs. adeno)
1.10
0.12–9.88
0.935
1.68
0.16–17.36
0.665
Therapy line
0.80
0.09–6.75
0.838
4.00
0.18–90.51
0.384
PD-L1 TPS
1.00
0.94–1.05
0.906
1.00
0.94–1.07
0.925
miRisk (high vs. low)
1.41
0.17–11.91
0.754
1.17
0.13–10.82
0.889
Note: ECOG performance status, therapy line, and PD-L1 TPS were modeled as continuous variables. Histologic subtype and miRisk were modeled as categorical variables.
Currently, both IO and ICT are recommended as treatment options for patients with stage IV NSCLC and PD-L1 TPS greater than or equal to 50%. Nevertheless, the response rate to IO monotherapy is only 40%,
This additional therapeutic burden comes at the cost of increased frequency and severity of toxicity, with grade 3 to 4 adverse events noted in approximately 70% of patients.
Because there is currently no reliable biomarker to predict response to therapy, the decision to treat with IO versus ICT is largely based on clinical judgment, considering factors such as general health status, number of metastatic sites, and disease aggressiveness. Still, the optimal therapy for a given patient often remains unclear.
There is great interest in the discovery of blood-based biomarkers that are predictive of immunotherapy response due to their ease of noninvasive collection and their potential to capture signal both from the peripheral immune system and material shed from the tumor itself.
were found to be promising; however, these are yet to be approved for clinical use. Immune cell RNA profiling and next-generation sequencing-based methods could enable improved prediction with additional integration of signal from multiple sources, such as peripheral effector cell counts.
Encouraging results have been reported from the measurement of circulating miRNAs, known to be master regulators of gene expression, and implicated in multiple processes in immune regulation and cancer.
The miRisk score exploits these opportunities as a multivariable model that measures the expression of predominantly myeloid-derived miRNAs with predicted interactions with the PD-(L)1 signaling pathway.
This blood-based biomarker is found to have robust generalizable performance in a validation cohort for survival prediction after IO treatment (HR = 5.24, 95% CI: 2.17–12.66, p < 0.001) and utility as a complementary diagnostic for the decision to treat miRisk-high patients with ICT (HR = 0.35, 95% CI: 0.15–0.82, p = 0.018).
There are limitations to the current study. There was no randomization of treatment between the IO- and ICT-treated cohorts. Therefore, despite controlling for known confounders with multivariable analyses, it is impossible to rule out the influence of potential hidden confounders. We further acknowledge a lack of ethnic diversity in study participants. We aim to address these issues in an upcoming prospective clinical trial.
In summary, the therapeutic landscape in advanced NSCLC is rapidly developing, in large part due to the successes of immunotherapies. The only currently used biomarker, PD-L1 TPS, has several limitations, including poor predictive performance, the necessity for an invasive tissue biopsy, and the subsequent exposure to sampling bias because of tumor heterogeneity and different assay platforms.
As a result, there is an unmet need for more accurate and noninvasive diagnostics to guide treatment decisions. The miRisk score represents an immune-focused biomarker that is specifically predictive of response to immunotherapy and could serve as the foundation for a complementary diagnostic to guide therapeutic decisions and thereby allow physicians to more accurately choose between treating patients with IO alone versus with ICT.
Rastislav Horos, Paul Kittner, Mustafa Kahraman, Tobias Sikosek: Investigation, Formal analysis, Writing—review and editing.
Franziska Hinkfoth, Kaja Tikk: Data curation, Writing—review and editing.
Nathaniel D. Mercaldo: Formal analysis, Writing—review and editing.
Albrecht Stenzinger, Klaus F. Rabe, Martin Reck, Michael Thomas: Data curation, Investigation, Formal analysis, Writing—review and editing.
Petros Christopoulos: Conceptualization, Data curation, Investigation, Formal analysis, Writing—original draft, Writing—review and editing.
Bruno R. Steinkraus: Conceptualization, Investigation, Formal analysis, Writing—original draft, Writing—review and editing.
Acknowledgments
The authors thank Stephanie Axenfeld, Urszula Jagos-Ihde, Claudia Genest, Marlen Szewczyk, Milena Schmidt, and all other contributing medical personnel at the LungenClinic in Grosshansdorf for their outstanding work with patient enrollment and data collection. The authors also thank Elena Gleim and the entire laboratory team at Hummingbird Diagnostics GmbH for their excellent technical assistance during the study conduct. The authors thank Bruce Johnson for feedback on the manuscript.
Association of the advanced lung cancer inflammation index (ALI) with immune checkpoint inhibitor efficacy in patients with advanced non-small-cell lung cancer.
Disclosure: Prof. Christopoulos declares receiving research funding from AstraZeneca, Novartis, Roche, and Takeda and advisory board, lecture, or educational fees from AstraZeneca, Boehringer Ingelheim, Chugai, Kite, Novartis, Pfizer, Roche, and Takeda. Prof. Reck reports receiving honoraria for lectures and consultancy from AstraZeneca, Amgen, Bristol-Myers Squibb, Boehringer Ingelheim, Eli Lilly, Merck, Merck Sharp & Dohme, Mirati, Novartis, Sanofi, Pfizer, and Roche. Prof. Rabe reports receiving payments or honoraria from Boehringer Ingelheim, AstraZeneca, Novartis, Roche, Chiesi Pharmaceuticals, Regeneron, Sanofi, and Berlin Chemie outside of the submitted work. Prof. Thomas discloses receiving honoraria from AstraZeneca, Bristol-Myers Squibb, Boehringer Ingelheim, Celgene, Chugai, Eli Lilly, Merck Sharp & Dohme, Novartis, Pfizer, Roche, Takeda, Sanofi, Beigene, and GlaxoSmithKline and research funding from AstraZeneca, Bristol-Myers Squibb, Roche, and Takeda. Prof. Stenzinger reports having advisory board, or speaker’s bureau engagements with Aignostics, Amgen, AstraZeneca, Bayer, Bristol-Myers Squibb, Eli Lilly, Illumina, Incyte, Janssen, Merck Sharp & Dohme, Novartis, Pfizer, Roche, Seattle Genetics, Takeda, and Thermo Fisher and receiving grants from Bayer, Bristol-Myers Squibb, Chugai, and Incyte. Dr. Rajakumar, Dr. Horos, Mr. Kittner, Dr. Kahraman, Dr. Sikosek, Ms. Hinkfoth, Dr. Tikk and Dr. Steinkraus are employees of Hummingbird Diagnostics and hold company stock options. Dr. Rajakumar, Dr. Horos, Dr. Sikosek, and Dr. Steinkraus are inventors of patent applications related to response prediction for immunotherapy submitted by Hummingbird Diagnostics. Prof. Christopoulos and Prof. Reck serve on the clinical advisory board of Hummingbird Diagnostics. Dr. Mercaldo reports receiving consulting fees from Hummingbird Diagnostics.
Cite this article as: Rajakumar T, Horos R, Kittner P, et al. Brief report: a blood-based microRNA complementary diagnostic predicts immunotherapy efficacy in advanced-stage NSCLC with high programmed death-ligand 1 expression. JTO Clin Res Rep. 2022;3:100369.
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